Physical characterization of ilv-lac fusions
نویسندگان
چکیده
منابع مشابه
Regulation of proline utilization in Salmonella typhimurium: characterization of put::Mu d(Ap, lac) operon fusions.
The genes for proline utilization were fused to the structural genes of the lac operon by use of the hybrid Mu phage derivative Mu d(Ap lac). Stable deletion derivatives of these fusions were selected and used to study the transcriptional regulation of the put genes. Analysis of these fusions showed that the putA gene product, a bifunctional oxidase-dehydrogenase, also serves to negatively cont...
متن کاملConstruction from Mu d1 (lac Apr) lysogens of lambda bacteriophage bearing promoter-lac fusions: isolation of lambda ppheA-lac.
Bacteriophage Mu d1 (lac Aprr) was used to obtain strains of Escherichia coli K-12 in which the lac genes are expressed from the promoter of pheA, the structural gene for the enzyme chorismate mutase P-prephenate-dehydratase. A derivative of bacteriophage lambda which carries the pheA-lac fusion was prepared; the method used is generally applicable for the construction, from Mu dl lysogens, of ...
متن کاملGenetic fusions defining trp and lac operon regulatory elements.
Two procedures for easily isolating deletions that fuse the trp and lac operons are described. Using these procedures, a large number of fusion deletions have been isolated. The 1a.c ends of these deletions extend varying distances into the &I gene and the Z&c promoter-operator region. Therefore, contrary to a previous report, there does not appear to be a messenger-termination signal at the Ct...
متن کاملFusions of the lac and trp Regions of the Escherichia coli Chromosome.
Two classes of strains were studied in which the lac operon is transposed to a chromosomal site close to the tonB and trp loci. The two classes differ in the orientation of the lac region on the chromosome. In both types of strains, tonB mutants were selected in which deletions removing the tonB locus also caused a fusion of the lac and trp regions. The study of the properties of such fusion st...
متن کاملFusions of the lac and trp regions of escherichia coli: covalently fused messenger RNA.
A bacterial strain is described in which the structural genes of the lac operon have been fused by deletion to the trp operon. It is shown that in this strain lac messenger RNA synthesis is controlled by the t?y regulatory gene. DNA-RNA hybridization studies show that a fraction of the lac messenger RNA is covalently linked to the tr2, messenger RNA. These results demonstrate that at least a fr...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1979
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.140.1.251-260.1979